Experiment+Report+D_Enzyme+action

EXPERIMENT REPORT- INVESTIGATING THE EFFECT OF TEMPERATURE AND pH ON THE ENZYME REACTION

PART A

Results **Testube** || **Contents** || **Iodine Test** || **Conclusion** || A1 || 5cm3 of starch solution + 3cm3 of amylase placed in a beaker of ice water || the mixture turned blue-black in colour || the starch digestion has not occurred || B1 || 5cm3 of starch solution + 3cm3 of amylase placed in a watch bath of 37 degree celsius || the mixture turned blue-black in colour || the starch digestion has not occurred || C1 || 5cm3 of starch solution + 3cm3 of amylase placed in boiling water || the mixture turned brown in colour || the starch digestion has occurred || D1 || 5cm3 of starch solution + 3cm3 of distilled water placed in a watch bath of 37 degree celsius || the mixture turned blue-black in colour || the starch digestion has not occurred ||

Interpretation

Test tube C1 shows that starch digestion has occurred as it is the only mixture that reacted with the iodine to turn brown in colour. The other test-tubes, A1, B1, D1 contained mixtures that gave a blue-black colour. The amylase in these test-tubes did not break down with the starch solution, hence the iodine solution is still able to react with the starch solution to give a blue-black colour. The reaction between the starch solution and amylase will produce carbohydrates, which can be detected using the Bendict’s test. Test-tube D1 serves as a control for the experiment to indicate the colour for the reaction of the iodine with the starch solution. From this experiment, we can conclude that the higher the surrounding temperature, the higher the rate of reaction between amylase and starch solution

PART B

Results

**Test tube** || **Contents** || **Iodine Test** || **Conclusion** || W || 2cm3 of starch solution + 2cm3 of amylase + 2cm3 of HCL || the mixture turned blue-black in colour || the starch digestion has not occurred || X || 2cm3 of starch solution + 2cm3 of amylase + 2cm3 of distilled water || the mixture turned brown in colour || the starch digestion has occurred || Y || 2cm3 of starch solution + 2cm3 of amylase + 2cm3 of NH4OH || the mixture turned blue-black in colour || the starch digestion has not occurred || Z || 2cm3 of starch solution + 4cm3 of distilled water || the mixture turned blue-black in colour || the starch digestion has not occurred ||

Interpretation

Test tube X shows that starch digestion has occurred as it is the only mixture that reacted with the iodine to turn brown in colour. The other test-tubes, W, Y, Z contained mixtures that gave a blue-black colour. The amylase in these test-tubes did not break down with the starch solution, hence the iodine solution is still able to react with the starch solution to give a blue-black colour. Test-tube Z serves as a control for the experiment to indicate the colour for the reaction of the iodine with the starch solution. From this experiment, we can conclude that the rate of reaction between starch solution and amylase is the highest when there is a neutral pH level.